GATE 2025 Syllabus for Biotechnology (BT): Download Syllabus with Weightage PDF

The Indian Institute of Technology Roorkee will conduct the GATE Biotechnology Exam 2025, providing admission to ME/M. Tech/Ph.D. courses and job opportunities. The Syllabus for the GATE has been released on the official website at gate.iitr.ac.in. The syllabus is divided into three parts: General Aptitude, Engineering Mathematics, and Core Discipline which in this case will be Biotechnology or BT.

The General Aptitude section, common to all GATE exams, carries a 15% weightage, Engineering Mathematics will hold a weightage of 13% weightage while the Core Discipline or Biotechnology subjects hold a 72% weightage. Aspirants should make themselves familiar with the complete biotechnology syllabus to manage their study plans effectively and focus on key topics as the exam date approaches.

The GATE BT Syllabus 2025 outlines the topic-wise details of all subjects that may appear in the exam. The exam is expected to be conducted in February 1-9, 2025, with registrations starting in August 2024. While the syllabus can be covered in a minimum of six months, however, it is always recommended to start early preparation.

Syllabus PDF
General Aptitude Syllabus Download PDF
Engineering Mathematics + Biotechnology Syllabus Download PDF
GATE BT Subject Wise Weightage

GATE BT Subject Wise Marks Weightage

Important Topics Expected Marks/ Weightage
General Aptitude 15
Section 1: Engineering Mathematics 13
Section 2: General Biology 23
Section 3: Genetics, Cellular and Molecular Biology 21
Section 4: Fundamentals of Biological Engineering 10
Section 5: Bioprocess Engineering and Process Biotechnology 7
Section 6: Plant, Animal and Microbial Biotechnology 6
Section 7: Recombinant DNA Technology and Other Tools in Biotechnology 5
TOTAL MARKS 100

GATE BT Highlights

GATE BT in a Nutshell

Biotechnology Paper Code BT
Exam Mode Online
Total Marks 100
Number of Questions 65
Types of Questions MCQs, MSQs, and NAT
Number of Sections General Aptitude (GA) + Candidate's Selected Subjects
Weightage by Section

Section I :
General Aptitude – 15% of total marks

Section II :
Engineering Mathematics – 13% of total marks
Biotechnology Subjects – 72% of total marks

Total Time Duration 3 hours (180 minutes)

GATE BT Important Sections

Important Sections to Note

Section 1
Numerical Computation English Grammar
Numerical Estimation Verbal Analogies
Numerical Reasoning and Data Interpretation Vocabulary
Section 2
Linear Algebra Complex Analysis
Calculus Numerical Methods
Differential Equations ( ODE and PDE) Probability and Statistics
Vector Analysis Limits and Continuity
Section 3
General Biology Bioprocess Engineering and Process Biotechnology
Genetics, Cellular and Molecular Biology Plant, Animal and Microbial Biotechnology
Fundamentals of Biological Engineering Recombinant DNA Technology and Other Tools in Biotechnology

GATE BT Preparation Tips

Tips to Crack GATE BT 2025

Candidates who are preparing for the GATE Biotechnology exam definitely need a strategic approach to ace GATE BT 2025. Here are some valuable tips from the top rankers that can help you succeed:

  • Use Previous Year Book (Geetha): Try to solve the previous year's question paper a minimum of three times as follows : At first, try to solve every question while reading the subject for the first time. The second time, solve the book during revision. Finally, focus on star-marked questions. If time permits, solve the entire book again.
  • Supplementary Workbooks: Candidates can solve the supplementary workbooks such as Solve Made Easy Workbook first as it has a good collection of questions. If possible to get along the tougher side of the question, also solve Bits and Bytes.
  • Make Short Notes: Try to Include all necessary formulas, concepts, and unit conversions in your notes, and remember to update them regularly as you study.
  • Practice with a Stopwatch: Gate Toppers always prefer to time themselves to build speed and accuracy, crucial for exams like CIL, ISRO, BARC, ESE, SSC JE, and NLC. Although GATE provides sufficient time, other exams do not.
  • Limit Textbook Reading: GATE BT Aspirants shouldn’t rely heavily on standard textbooks unless necessary. Avoid lengthy textbook questions not relevant to GATE.
  • Complete Test Series: After completion of revision start the test series in July. Give part tests after the first revision. Follow with subject-wise and multi-subject tests after the second revision. Begin full-length tests from December, aiming for 20-30 FLTs.

GATE BT Previous Year Question Papers

Previous Year Question Papers for Biotechnology (BT)

Session Question Paper PDF
2024 Check Here
2023 Check Here
2022 Check Here
2021 Check Here
2020 Check Here

GATE BT Books

Books to refer to for GATE Biotechnology 2025

Books Publishers/ Authors
Principles of Biochemistry Lehninger
Molecular Cell Biology Iodish and Baltimore
Biotechnology Pranav Kumar
GATE General Aptitude and Mathematics GK publishers
Immunology Kuby
Gene Cloning and DNA analysis T.A Brown
Bioprocess Engineering Principles P.Doran
Advanced Engineering Mathematics Erwin Kreyszig
Plant Biotech Slater
High School English Grammar Wren and Martin
A Modern Approach to Verbal and Non-Verbal Reasoning R.S. Aggarwal
How to Prepare for Quantitative Aptitude Arun Sharma
Previous Year Books Made Easy Workbook or Bits And Bytes or Both

Frequently Asked Questions

Frequently Asked Questions

Ques. When will the official syllabus for GATE BT will be declared by IIT Roorkee?

Ans. GATE Syllabus of the year 2025 has been released on the official website of gate2025.iitr.ac.in on July 1, 2024.

Ques. How much time is required to cover the entire GATE Syllabus for BT in 2025?

Ans. On Average, candidates take at least 6 months to complete the GATE Syllabus for BT. It is always advisable to start the preparation a bit early, that will help aspirants in revising the syllabus prior to the examination.


*The article might have information for the previous academic years, which will be updated soon subject to the notification issued by the University/College.

GATE 2025 : 5 Answered Questions

Ques. What are the cut off marks in gate for iit indore?

IIT Indore GATE Cutoss 2023 has not been released officially IIT Kanpur will release the GATE 2023 cutoff for IIT Indore Students having valid GATE scores from 2023, 2022, or 2021 will be eligible for admission to MTech courses. Considering the IIT Indore GATE cutoff for 2021, The overall cutoff for GATE is 615 - 740 marks. Here is the branch-wise cutoff M.Tech Material Science and Engineering 615 M.Tech Communication and Signal Processing 657 M.Tech VLSI Design and Nanoelectronics 670 M.Tech Production & Industrial Engineering 740 Please note that the above cutoff is only for the general category....Read More
Answer By Jyoti Chopra 17 Jun 23
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Ques. Is it possible to get into IIT Tirupati just by the GATE qualifying marks?

Yes, it may be possible. Here are the reasons why- IIT Tirupati is a very new IIT, established in 2015. It is not well known currently, especially in North India.  It releases its applications quite early. Its MS and Phd. batches started only a few years ago, so those with better scores will prefer NITs over IIT Tirupati. The cutoff for MS is also low in IIT Madras because very few students are interested in pursuing the course. Ultimately, the number of applicants and the interview round will decide your fate. ...Read More
Answer By Divya Saraf 11 Jan 24
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Ques. Can Longterm students apply gitam gate exam?

Verify your eligibility for your desired course on the GITAM website or get in touch with the admissions office directly if you are a "longterm student"—that is, if you finished your qualifying exam several years ago. Unless otherwise noted, most programmes do not specifically exclude long-term students. Student Type Eligible for GITAM GAT? Notes/Clarification Appearing in the current year Yes For example, 12th in 2025 or graduation in 2025 Passed in the previous year Usually Yes No explicit gap year limit for most courses Long-term gap (many years) Check course-wise criteria No strict age/gap limit, but confirm for each program Yes, long-term students can generally apply for the GITAM GAT (GITAM Admission Test) exam, provided they meet the eligibility criteria for the specific course they are interested in....Read More
Answer By Vinima Bhola 23 Jun 25
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Ques. My son has scored 42.33 marks in GATE (CSE). Could you please suggest which IITs, NITs, or IIITs he might be eligible for an M.Tech in Computer Science?

With a GATE 2025 score of 42.33 marks in Computer Science, your son has comfortably crossed the General category qualifying cut-off mark of 29.2 marks and can be eligible for admission into a number of top institutes in India. Here is a quick analysis of where he stands and the options that actually confront him: Admission Possibilities Institute Type Chance for Admission in CSE Top IITs (Bombay, Delhi, Kanpur, etc.) Unlikely, as cutoffs are usually 55–65+ marks Newer IITs (Jodhpur, Patna, Dhanbad, etc.) Possible in later rounds or for allied CSE branches Top NITs (Trichy, Warangal, Surathkal) Competitive, but admission more likely in later rounds or for specializations like information security and data science. Mid/Lower NITs Good chance for direct admission in CSE Top IIITs (Allahabad, Gwalior, Jabalpur) Competitive; possible depending on the round and specialization Other IIITs Good chances for CSE or related programs Recommendations: Apply through COAP (for IITs) and CCMT (for NITs/IIITs). Keep looking for related fields like Data Science, Cybersecurity, or Information Technology, which may have slightly lower cutoffs. And take all rounds of counseling, including spot rounds, as cutoff trends fluctuate every year. Also visit state universities and centrally funded colleges that accept GATE scores. Your son is rightly positioned for M.Tech admissions in all NITs, IIITs, and new IITs. CSE and this score will not make the top 5 IITs feasible, but there are plenty of good colleges where he can build a strong academic and professional life. Wishing your son best of luck for future admissions....Read More
Answer By Muskan Agrahari 24 Jun 25
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Ques. I have completed my M.Tech with 73% marks but have not qualified for GATE. Am I eligible to apply for full-time Ph.D. admission at IIT Bhubaneswar?

Eligibility for Full-time Ph.D. at IIT Bhubaneswar (Engineering/Science) Criteria Requirement M.Tech. Percentage Minimum 60% marks (you have 73%, so you meet this criterion) GATE Qualification Required for Institute PhD Scholar (regular full-time, with assistantship) GATE Waiver Only for sponsored projects or special categories With 73% in M.Tech, you are academically eligible, but for regular full-time Ph.D. admission (institute-sponsored) in IIT Bhubaneswar, a valid GATE score is required. But you can still apply under sponsored, project, or special categories, where GATE is not required, but other requirements (such as work experience or organizational sponsorship) are there....Read More
Answer By Muskan Agrahari 24 Jun 25
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Fees Structure

Structure based on different categories

CategoriesState
General1800
Women900
sc900
pwd900

Note: GATE 2024 Application Fee needs to be paid online through net banking or debit card or credit card facilities. Additional charges will be applicable as per the rule of the bank from where the money is being transferred. This charge will be specified on the payment portal.

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