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CUET PG Biochemistry Question Paper 2025 is available here for download. NTA conducted CUET PG Biochemistry paper 2025 on March 16 in Shift 1. CUET PG Question Paper 2025 is based on objective-type questions (MCQs). According to latest exam pattern, candidates get 90 minutes to solve 75 MCQs in CUET PG 2025 Biochemistry question paper.
CUET PG Biochemistry Question Paper 2025 PDF with Solution PDF
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CUET PG 2025 Biochemistry Questions with Solutions
Question 1.
Who is the father of microbiology?
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The father of microbiology is Louis Pasteur, a French biologist and chemist who made groundbreaking contributions to the study of microorganisms. He is best known for his discoveries of the principles of vaccination, microbial fermentation, and pasteurization. His work laid the foundation for the field of microbiology.Louis Pasteur's significant achievements include:
- Germ Theory of Disease: Pasteur's research demonstrated that microorganisms were the cause of many diseases, revolutionizing medicine and public health.
- Pasteurization: Pasteur developed pasteurization to kill harmful bacteria without damaging liquids like milk and wine.
- Vaccination Development: Pasteur is credited with developing vaccines for rabies and anthrax, saving millions of lives.
Question 2.
What is the PCR sequence?
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Polymerase Chain Reaction (PCR) is a molecular biology technique used to amplify specific segments of DNA. It allows researchers to produce millions of copies of a DNA segment from a small sample. PCR has been instrumental in numerous fields, including genetics, diagnostics, and forensic science. The PCR process consists of three main steps:1. Denaturation: The double-stranded DNA template is heated to 94–98°C, breaking the hydrogen bonds between complementary bases, resulting in two single strands.
2. Annealing: The reaction temperature is lowered to 50–65°C to allow short DNA primers to bind to the complementary sequences at the 3' ends of the target DNA region.
3. Extension (Elongation): The temperature is raised to around 75–80°C, allowing the DNA polymerase enzyme to add nucleotides and form a new strand of DNA complementary to the original strand.
Question 3.
What is the composition of a lipid raft?
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Lipid rafts are specialized microdomains in the cell membrane enriched with cholesterol, sphingolipids, and specific proteins. These rafts act as platforms for signaling molecules, affecting cell signaling, membrane trafficking, and protein sorting.Key Components:
- Cholesterol: Provides structural integrity, reducing membrane fluidity, and helps stabilize the raft structure.
- Sphingolipids: Contribute to the raft's ordered structure and play a role in organizing lipid rafts.
- GPI-anchored proteins: Proteins that are covalently attached to the membrane through a GPI anchor, involved in signaling and cell adhesion.
Question 4.
Strict vegans lack which vitamin?
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Strict vegans are at risk of vitamin B12 deficiency as it is predominantly found in animal products. Since vegans avoid animal-based foods, they may need supplements or fortified foods to meet their vitamin B12 requirements.Question 5.
Which DNA makes a right-handed helix?
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A-DNA and B-DNA are both right-handed helices. B-DNA is the most common form under physiological conditions, whereas A-DNA forms under dehydrating conditions.Question 6.
Which DNA makes a left-handed helix?
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Z-DNA is a left-handed helix, with a zigzag backbone. It forms under specific conditions such as high salt concentrations or negative supercoiling.Question 7.
What is the most stable form of glucose?
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The most stable form of glucose is the chair conformation. In the chair conformation, the glucose molecule adopts a stable, three-dimensional form that minimizes steric clashes between atoms. This form is the most energetically favorable and commonly found in nature, particularly in polysaccharides like starch and cellulose.Question 8.
What is the linkage in amylose?
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Amylose consists of glucose units linked by 1-4 glycosidic bonds. This linkage is between the first carbon of one glucose molecule and the fourth carbon of the next. Amylose is a component of starch and forms a helical structure due to the 1-4 linkage.Question 9.
What is involved in substrate-level phosphorylation?
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Substrate-level phosphorylation involves the transfer of a phosphate group from a high-energy substrate molecule to ADP to form ATP. Enzymes such as pyruvate kinase and phosphoglycerate kinase are involved in this process. These enzymes catalyze reactions where a phosphate group is directly transferred to ADP, bypassing the need for the electron transport chain.Question 10.
After KDEL mutation, it became KEEL. Where did the mutation happen?
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The mutation in the KDEL sequence occurred at the third position, where the amino acid Aspartic acid (D) was replaced by Glutamic acid (E). The KDEL sequence is a C-terminal signal that helps proteins return to the endoplasmic reticulum from the Golgi apparatus. The mutation from D to E affects the protein's recognition and transport function.
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