CUET PG Life Science 2025 Question Paper (Available): Download Question Paper with Answer Key And Solutions PDF

To observe the specific part of a tissue section under compound microscope, the contrast can be increased by:

• (A) Staining
• (B) Changing magnification of the microscope
• (C) Changing microscope resolution
• (D) Use of fluorescence dyes

Which of the following will be the part of the pellet, when tissue cells are homogenized and centrifuged at 1000g for 10 min.?

• (A) Microsomes
• (B) Mitochondria
• (C) Nuclei
• (D) Ribosomes

Arrange the following DNA fragments A, B, C, D separated after Gel Electrophoresis in increasing order of number of their base pairs

• (A) A, B, C, D
• (B) A, D, C, B
• (C) B, C, D, A
• (D) C, B, D, A

Which of the following is not true about gel electrophoresis?

• (A) Gel electrophoresis can be used to separate nucleic acids or proteins.
• (B) Agarose and polyacrylamide are routinely used in gel electrophoresis.
• (C) In gel electrophoresis of DNA, the digested DNA fragments move toward the negative electrode of the tank.
• (D) In gel electrophoresis of DNA, ethidium bromide, a DNA binding dye, is used to locate the DNA fragments of interest under UV light.

Which of the following are the types of column chromatography?

A. Paper chromatography
B. Affinity chromatography
C. Size exclusion chromatography
D. Ion-exchange chromatography

Choose the correct answer from the options given below:

• (A) A, B and D only
• (B) A, B and C only
• (C) A, B, C and D
• (D) B, C and D only

Tryptophan, tyrosine and phenylalanine, absorb ultraviolet light. Therefore, the protein rich in these amino acids strongly absorbs the light of wavelength ___________ due to aromatic side chain of these amino acids.

• (A) 90 nm
• (B) 420 nm
• (C) 550 nm
• (D) 280 nm

A scientist wants to separate three protein molecules by ion-exchange chromatography. The pH of the mobile phase of the column is maintained in such a way that the protein molecule (A) has a net charge of -2, protein molecule (B) has a net charge of +2 and protein molecule (C) has a net charge of +1. Which one of the molecule/s will elute first from a cation-exchange resin?

• (A) Protein molecule A
• (B) Protein molecule B
• (C) Protein molecule C
• (D) All protein molecules elute simultaneously

A technique for transferring denatured DNA molecules that have been separated electrophoretically from a gel to a matrix (such as a nitrocellulose or nylon membrane) on which a hybridization assay can be performed is called

• (A) Northern blotting
• (B) Southern blotting
• (C) Eastern blotting
• (D) Western blotting

The DNA of the bacterial cell is protected from the cell's own restriction enzymes by the addition of methyl group (-CH3) to ___________ of cytosine.

A. 4th Carbon
B. 7th Carbon
C. 6th Carbon
D. 5th Carbon

Choose the correct answer from the options given below:

• (A) D only
• (B) B only
• (C) B and D only
• (D) B and C only

Which of the following statements are correct regarding prokaryotic genomic organization?

A. Most prokaryotes contain a single large circle of double stranded DNA as its genome.
B. The nucleoid is an irregularly shaped region that contains the cell's chromosome and numerous proteins.
C. Nucleoid associated proteins (NAPs) are not particularly important during cell division for the compaction of chromosome.
D. Prokaryotic genomes are much smaller as compared to eukaryotes.

Choose the correct answer from the options given below:

• (A) A, B and D only
• (B) A, B and C only
• (C) A, B, C and D
• (D) B, C and D only

In humans, one of the most recognized satellite DNA sequences, found mainly in the centromere region is:

• (A) Quinoid family
• (B) Deltoid family
• (C) Alphoid family
• (D) Trepizoid family

Select correct statement about Euchromatin :

• (A) Highly condensed
• (B) Associated with active transcription
• (C) Found outside nucleus only
• (D) Does not replicate during S phase

Match the LIST-I with LIST-II

Choose the correct answer from the options given below:

• (A) A-I, B - II, C - III, D - IV
• (B) A-I, B - III, C - II, D - IV
• (C) A-III, B - I, C - IV, D - II
• (D) A-III, B - IV, C - I, D - II

Telomerase acts as a cellular reverse transcriptase that provides the active site for:

• (A) RNA-dependent DNA synthesis
• (B) DNA-dependent RNA synthesis
• (C) RNA-dependent RNA synthesis
• (D) DNA-dependent DNA synthesis

Which of the following molecule/ion is not transported/buffered by hemoglobin?

• (A) O\(_2\)
• (B) CO\(_2\)
• (C) Na\(^+\)
• (D) H\(^+\)

Which of the following pair of class and organism is not correctly matched?

• (A) Polyplacophora- Chiton
• (B) Gastropoda - Aplysia
• (C) Monoplacophora - Chiton
• (D) Aplacophora - Chaetoderma

Which one of the following statement is incorrect regarding the work of Erwin Chargaff?

• (A) The base composition of DNA don't varies from one species to another.
• (B) DNA specimens isolated from different tissues of the same species have the same base composition.
• (C) The base composition of DNA in a given species does not change with an organism's age, nutritional state, or changing environment.
• (D) The sum of the purine residues equals the sum of the pyrimidine residues; that is, A + G = T + C.

Which of the following amino acids are essential amino acids in humans:

A. Leucine
B. Lysine
C. Isoleucine
D. Serine

Choose the correct answer from the options given below:

• (A) A, B, and C Only
• (B) B, C, and D Only
• (C) A, B, C and D
• (D) A, C, and D Only

Match LIST-I with LIST-II

Choose the correct answer from the options given below:

• (A) A-I, B-III, C - IV, D - II
• (B) A - III, B - II, C - IV, D - I
• (C) A-I, B-II, C - IV, D - III
• (D) A-III, B - IV, C - I, D - II

The glycosidic bond between the monomers of sucrose is-

• (A) Gal(1\(\beta \rightarrow \beta\)4)Glc
• (B) Fru(4\(\beta \rightarrow \beta\)1)Glc
• (C) Fru(4\(\beta \rightarrow \alpha\)2)Glc
• (D) Fru(2\(\beta \leftrightarrow \alpha\)1)Glc

Match LIST-I with LIST-II

Choose the correct answer from the options given below:

• (A) A - IV, B - III, C - I, D - II
• (B) A - IV, B - III, C - II, D - I
• (C) A - I, B - II, C - IV, D - III
• (D) A - III, B - IV, C - I, D - II

Arrange the steps of recombinant DNA-cloning procedure in correct sequence of their occurance:

A. Joining the target DNA with cloning vector DNA by ligase enzyme.
B. Targeted DNA from a source organism is cleaved and cloning vector DNA is also cleaved by the same restriction endonuclease.
C. Selection of transformants from non-transformants through antibiotic resistance and color indicators
D. Introduction of recombinant DNA into host cell.

Choose the correct answer from the options given below:

• (A) A, B, C, D
• (B) A, C, B, D
• (C) B, A, D, C
• (D) C, B, D, A

Tetracycline is usually mixed with the culturing medium as selective agents in selecting transformants in gene cloning technique. It acts by:

• (A) Inhibiting cell wall formation; inactivated by \(\beta\)-lactamase
• (B) Binding to the 30S ribosomal subunit and inhibits the binding of aminoacyl-tRNAs in bacteria
• (C) Blocking protein initiation complex formation and causes misreading during translation
• (D) Binding to 50S ribosomal subunit and inhibits protein synthesis.

Match LIST-I with LIST-II

Choose the correct answer from the options given below:

• (A) A - II, B - III, C - IV, D - I
• (B) A-I, B-III, C - II, D - IV
• (C) A - II, B - IV, C - I, D - III
• (D) A-III, B - IV, C - I, D - II

For sterilization, the culture media and glasswares must be generally autoclaved at:

• (A) 121\(^\circ\)C and 19 psi
• (B) 121\(^\circ\)C and 15 psi
• (C) 112\(^\circ\)C and 15 psi
• (D) 120\(^\circ\)C and 20 psi

Benzoic acid or benzoates are used to preserve food from

A. Insects
B. Yeast
C. Clostridia
D. Molds

Choose the correct answer from the options given below:

• (A) A and B only
• (B) B and C only
• (C) A, C and D only
• (D) B and D only

The Bdellovibrios bacteria is a _________ bacteria:

A. Gram negative
B. Predator of gram negative bacteria
C. It has a single polar flagellum
D. Reproduce by producing spores called myxospore during nutrient deficit conditions

Choose the correct answer from the options given below:

• (A) A, B and D only
• (B) A, B and C only
• (C) A, B, C and D
• (D) B, C and D only

Organisms belongs to the genus bacteroides are:

• (A) Chemoautotrophs, aerobic and gram positive.
• (B) Causative agents of Lyme diseases.
• (C) Anaerobic, gram-negative, nonspore-forming rods and usually nonmotile.
• (D) Not able to degrade cellulose, pectins, and other complex carbohydrates.

A method for culturing anaerobes is the GasPak System, which uses hydrogen and a ___________ catalyst to remove O\(_2\).

• (A) Rubidium
• (B) Lead
• (C) Iridium
• (D) Palladium

The antimicrobial agent mercuric chloride functions by

• (A) Interfering with cellular oxidation process.
• (B) Inhibiting the enzymes which contain the sulfhydryl group.
• (C) Alkylation reactions with organic compounds such as enzymes and other proteins.
• (D) Denaturation of proteins, interference with glycolysis and membrane damage.

Match LIST-I with LIST-II

Choose the correct answer from the options given below:

• (A) A-I, B-II, C-III, D-IV
• (B) A-I, B-III, C-II, D-IV
• (C) A-I, B-II, C-IV, D-III
• (D) A-III, B-IV, C-I, D-II

The Ti plasmid and its T-DNA have been genetically modified for use as a vector for the insertion of recombinant DNA into plant chromosomes to prevent dicots from tumor diseases. The source of Ti plasmid is:

• (A) Asparagus sp.
• (B) Arthrobacter luteus
• (C) Aspergillus niger
• (D) Agrobacterium tumefaciens

The ability of a molecule to function as an antigen depends on its:

A. Size
B. Structural complexity
C. Chemical nature
D. Degree of foreign nature to the host

Choose the correct answer from the options given below:

• (A) A, B and D only
• (B) A, B and C only
• (C) A, B, C and D
• (D) B, C and D only

Match LIST-I with LIST-II

Choose the correct answer from the options given below:

• (A) A-I, B-II, C - III, D - IV
• (B) A-I, B - III, C - II, D - IV
• (C) A-I, BII, C - IV, D - III
• (D) A-III, B - IV, C - I, D - II

The cationic antimicrobial peptide, histatin is found in human's_____________.

• (A) Synovial fluid
• (B) Cerebrospinal fluid
• (C) Bile juice
• (D) Saliva

Convalescent sera belongs to

• (A) Sera from healthy individual
• (B) Sera of patients chronically infected with bacterial disease
• (C) Sera of patients recovering from infectious disease
• (D) Sera of patients in the latent phase of infection

Typhoid Vi antigen type of vaccine is:

• (A) Bacterial products
• (B) Killed products
• (C) Live products
• (D) Subunit

Which one of the given statement is not correct about plant viruses ?

• (A) In viruses, the envelope proteins may even project from the envelope surface as spikes are also called as protomers
• (B) In viruses, the envelope proteins may even project from the envelope surface as spikes are also called as concatemers
• (C) In viruses, the envelope proteins may even project from the envelope surface as spikes are also called as peplomers.
• (D) In viruses, the envelope proteins may even project from the envelope surface as spikes are also called as capsomers

Hepatitis C virus can cause liver cancer. It has:

• (A) ds DNA genome
• (B) ssDNA genome
• (C) ss RNA genome
• (D) ds RNA genome

The role of slow sand filters in water treatment facilities is:

• (A) To remove water borne microbes upto a set standard.
• (B) To remove excess ammonia present in the polluted water
• (C) To evaluate the microbiological characteristics of water.
• (D) To neutralize the increased pH of the water.

Match LIST-I with LIST-II

Choose the correct answer from the options given below:

• (A) A-I, B-II, C - III, D - IV
• (B) A-II, B-I, C - IV, D - III
• (C) A-IV, B - III, C - II, D-I
• (D) A-III, B - IV, C - I, D - II

The F\(_1\) hybrid (Rr Ii) is crossed with a variety double recessive for both the traits. How many types of zygotes will be produced in the cross?

• (A) 1
• (B) 2
• (C) 3
• (D) 4

Which of the following statement is wrong about C\(_4\) plants

• (A) Kranz anatomy is present
• (B) Initial CO\(_2\) acceptor is Phosphoenol-pyruvate (PEP)
• (C) First stable product is 3-phosphoglycerate
• (D) Calvin cycle operates along with the Hatch and Slack cycle.

The plants bloom when the light duration is less than 12 hours per day are know as.

• (A) Short day plants
• (B) Long day plants
• (C) Mid day plants
• (D) Day neutral plants

A rock body through which ground water flows is called

• (A) Lake
• (B) Artesian well
• (C) Springs
• (D) Aquifer

Match LIST-I with LIST-II

Choose the correct answer from the options given below:

• (A) A-I, B- II, C - III, D - IV
• (B) A-I, B- III, C - II, D - IV
• (C) A-II, B-I, C - IV, D - III
• (D) A-III, B - IV, C - I, D - II

The left-handed helix, Z-DNA is 18 AA (1.8 nm) in diameter which contains _____________________.

• (A) 10 bp per turn
• (B) 11 bp per turn
• (C) 10.4 bp per turn
• (D) 12 bp per turn

Choose the correct sequence of events as they occur during a bacterial transcription

A. RNA polymerase sigma (\(\sigma\)) subunits recognizes promoters
B. DNA double helix unwind
C. Sigma (\(\sigma\)) subunit dissociates from the holoenzyme
D. Formation of hairpin secondary structure

Choose the correct answer from the options given below:

• (A) A, B, C, D
• (B) A, C, B, D
• (C) B, A, D, C
• (D) C, B, D, A

Choose the correct answer regarding the translation of mRNA to proteins when AUG is the start codon and codes for methionine.

• (A) All proteins have methionine as the first amino acids.
• (B) Methionine never added at the beginning of protein synthesis.
• (C) An enzyme can cleave the methionine amino acid from the polypeptide after synthesis.
• (D) Methionine fall off automatically from the polypeptide just after termination step of the protein synthesis.

Choose the correct sequence of different regions on a typical eukaryotic DNA segment when we go from the upstream to the downstream of a transcription start point.

A. Initiation site
B. CAAT box
C. Enhancer
D. TATA box

Choose the correct answer from the options given below:

• (A) B, D, A, C
• (B) A, C, B, D
• (C) B, A, D, C
• (D) C, B, D, A

Which one of the following acid is hydrophobic with non polar side chain?

• (A) Tyrosine
• (B) Proline
• (C) Glutamine
• (D) Serine

Choose the correct order of steps involved in nucleotide excision repair of damaged bases as their occur.

A. A fresh burst of DNA synthesis
B. Cuts are made on both the 3' side and the 5' side of the damaged area
C. The DNA is unwound producing a "bubble".
D. A DNA ligase covalent binds the fresh piece into the backbone.

Choose the correct answer from the options given below:

• (A) A, B, C, D
• (B) B, C, A, D
• (C) C, B, A, D
• (D) A, B, D, C

Match LIST-I with LIST-II

Choose the correct answer from the options given below:

• (A) A-I, B-II, C - III, D- IV
• (B) A-I, B - III, C - II, D - IV
• (C) A-I, B-II, C - IV, D - III
• (D) A-III, B - IV, C - I, D - II

Frequency of recombination between linked genes in Drosophila is affected by

A. Distance between genes
B. Sex of heterozygotes for linked genes
C. Age of female
D. Temperature

Choose the correct answer from the options given below:

• (A) A, B and D only
• (B) A, B and C only
• (C) A, B, C and D
• (D) B, C and D only

Match the LIST-I with LIST-II

Choose the correct answer from the options given below:

• (A) A-I, B - II, C - III, D - IV
• (B) A-II, B-I, C - III, D - IV
• (C) A-I, B-II, C - IV, D - III
• (D) A-II, B-I, C - IV, D - III

In general F\(_2\), F\(_3\) and the subsequent generations do not show segregation for a cytoplasmically inherited trait. This is because

• (A) The F\(_1\) individuals generally receive plasmagenes from one parent only.
• (B) The F\(_1\) individuals generally receive nuclear genes from one parent only.
• (C) The F\(_1\) individuals generally receive plasmagenes from both parents.
• (D) The F\(_1\) individuals generally receive nuclear genes from both parents.

Which of the given factors can affect frequency in Mendelian population?

A. Migration
B. Mutation
C. Selection
D. Random shift

Choose the correct answer from the options given below:

• (A) A, B and D only
• (B) A, B and C only
• (C) A, B, C and D
• (D) B, C and D only

In 1963, several varieties such as Sonalika and Kalyan Sona were introduced all over the wheat-growing belt in India. They were

• (A) High yielding and disease susceptible
• (B) Low yielding and disease susceptible
• (C) High yielding and disease resistant
• (D) Low yielding and disease resistant

Among the following species of bryophytes, which is not an example of mosses?

• (A) Funaria
• (B) Marchantia
• (C) Polytrichum
• (D) Sphagnum

Who among the following is known as the father of Indian paleobotany?

• (A) Prof. Bhishma Sahni
• (B) Prof. Balraj Sahni
• (C) Prof. Birbal Sahni
• (D) Prof. Birpal Sahni

The symmetry in which a flower can be divided into two equal and similar halves by only one vertical division is

• (A) Actinomorphic
• (B) Polymorphic
• (C) Metamorphic
• (D) Zygomorphic

In homosporous pteridophyte species, the development of gametophyte is

• (A) Hemisporic
• (B) Episporic
• (C) Endosporic
• (D) Exosporic

More recently discovered plant hormone, Jasmonates, play an important role both in plant defense and development, is derived from which fatty acid?

• (A) Palmitic acid
• (B) Oleic acid
• (C) Linoleic acid
• (D) Linolenic acid

Plants can assimilate only two inorganic forms of nitrogen. These are:

A. Nitrite (NO\(_2^-\))
B. Ammonium (NH\(_4^+\))
C. Nitric acid (HNO\(_3\))
D. Nitrate (NO\(_3^-\))

Choose the correct answer from the options given below:

• (A) A and D only
• (B) B and D only
• (C) B, C and D only
• (D) A, B and D only

Lenticels permit the:

• (A) Exchange of water between the outer atmosphere and internal tissue of the stem in most woody trees.
• (B) Exchange of gases between the outer atmosphere and internal tissue of the stem in most woody trees.
• (C) Exchange of heat between the outer atmosphere and internal tissue of the stem in most woody trees.
• (D) Exchange of radiation between the outer atmosphere and internal tissue of the stem in most woody trees.

The membrane potential of a resting neuron is:

• (A) -20 and -60 millivolts
• (B) -30 and -80 millivolts
• (C) -20 and -80 millivolts
• (D) -60 and -80 millivolts

Which one of the following amino acids is involved in synthesis of hormone epinephrine in humans?

• (A) Threonine
• (B) Tryptophan
• (C) Tyrosine
• (D) Phenylalanine

Match LIST-I with LIST-II

Choose the correct answer from the options given below:

• (A) A - I, B - II, C - III, D - IV
• (B) A - I, B - III, C - II, D - IV
• (C) A - I, B - II, C - IV, D - III
• (D) A - III, B - IV, C - I, D - II

Which one of the following granulocyte has following features?
Highly phagocytic; nucleus with three to five lobes; primary and secondary granules; limited life span:

• (A) Eosinophil
• (B) Mast cells
• (C) Neutrophil
• (D) Basophil

Which of the following are autosomal recessive genetic disorder?

A. Huntington disease
B. Sickle cell anemia
C. Lesch-Nyhan syndrome
D. Tay-Sachs disease

Choose the correct answer from the options given below:

• (A) B and D only
• (B) A, B and C only
• (C) A, B, C and D
• (D) B, C and D only

Which one of the following proteins is often referred to as "the guardian of the genome"?

• (A) PFU
• (B) Rb
• (C) Myc
• (D) p53

Colibactin is a DNA-damaging compound and when taken up by human intestinal cells, colibactin result in double stranded DNA break and can cause human colorectal cancers. It is synthesized by:

• (A) Certain strain of E. coli
• (B) Helicobacter pylori
• (C) Bacteroides fragilis
• (D) Bifidobacterium spp.

Agar is used for preparing solid media for culture. Which of the following is not true about agar media?

• (A) Agar is obtain from a sea weed
• (B) It has virtually no nutritive value.
• (C) It melts at 98\(^\circ\)C and usually sets on 42\(^\circ\)C depending on agar concentration.
• (D) For solid media 0.2% to 0.5% agar concentration is employed.

Which one of the following groups of animal is devoid of gill slits?

• (A) Osteichthyes
• (B) Cyclostomata and Chondrichthyes
• (C) Echinodermata
• (D) Adult Amphibians

Which one of the following is not a tetrapods character?

• (A) Dermal scales
• (B) Neck and ribs
• (C) Pelvic girdle
• (D) Flat skull