Biotechnology is an important topic in the Biology section in MHT CET exam. Practising this topic will increase your score overall and make your conceptual grip on MHT CET exam stronger.
This article gives you a full set of MHT CET PYQs for Biotechnology with explanations for effective preparation. Practice of MHT CET Biology PYQs including Biotechnology questions regularly will improve accuracy, speed, and confidence in the MHT CET 2026 exam.
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MHT CET PYQs for Biotechnology with Solutions
1.
The maximum percentage of polyunsaturated fatty acid present in:- (A) Peanuts
- (B) Soybeans
- (C) Artificial butter
- (D) Palm oil
2.
ELISA is:- (A) Using radiolabelled second antibody.
- (B) Usage of RBCs.
- (C) Using complement-mediated cell lysis.
- (D) Addition of substrate that is converted into a coloured end product.
3.
The genetic material of M bacteriophage is _______- ds DNA
- ds RNA
- ss RNA
- ss DNA
4.
What is the main purpose of Polymerase Chain Reaction (PCR)?- To sequence DNA
- To amplify specific DNA sequences
- To cut DNA into fragments
- To identify mutations in genes
5.
Combination of foreign DNA and vector DNA are together called as _______.- Recombinant DNA
- Plasmid DNA
- DNA ligase
- Transformation DNA
6.
Why is it important to have iodized salt in our diet?- (A) It improves eyesight
- (B) It helps in the smooth functioning of the thyroid gland
- (C) It helps in the formation of bones
- (D) It helps to increase height
7.
Nephrons are connected with ______.- (A) Respiratory system
- (B) Nervous system
- (C) Circulatory system
- (D) Excretory system
8.
Absence of oxygen in earth, made the atmosphere reducing. But it later became oxidizing in nature due to the emergence of ________.- (A) Angiosperms
- (B) Gymnosperms
- (C) Cyanobacteria
- (D) Eukaryotic algae
9.
In genetic engineering the Foreign DNA is called- Recombinant DNA
- Exogenous DNA
- Mutated DNA
- Transferred DNA
10.
The cranial nerves which originate from the medulla oblongata are:- (A) IX, X, XI and XII
- (B) III, VIl, and IX
- (C) VII, VII IX and X
- (D) VIlI, IX, X, XI and XII
11.
In recombinant DNA technology, which enzyme is used to cut DNA at specific recognition sites to produce restriction fragments?- DNA polymerase
- Restriction endonuclease
- Ligase
- Reverse transcriptase
12.
Which one of the following is a decomposer?- (A) Fungi
- (B) Algae
- (C) Protozoa
- (D) Insect
13.
Respiration results in ___________.- (A) Release of oxygen
- (B) Anabolism
- (C) Release of carbon dioxide
- (D) Transfer of carbon dioxide
14.
The second heart sound during the cardiac cycle is produced by the:- (A) Simultaneous opening of the atrioventricular valves
- (B) Simultaneous closure of the atrioventricular valves
- (C) Simultaneous opening of the semilunar valves
- (D) Simultaneous closure of the semilunar valves
15.
Consider the following statements.A. Temperature is the most ecologically relevant environmental factor. B. Temperature increases progressively from the equator towards the poles and from plains to the mountain tops. C. Diversity is not an important characteristic of living organisms. D. Mango trees do not and cannot grow in temperate countries like Canada and Germany.Of the above statements:- (A) B and D are correct
- (B) A and B are correct
- (C) A and C are correct
- (D) A and D are correct



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