Applications of Biotechnology is an important topic in the Biology section in MHT CET exam. Practising this topic will increase your score overall and make your conceptual grip on MHT CET exam stronger.
This article gives you a full set of MHT CET PYQs for Applications of Biotechnology with explanations for effective preparation. Practice of MHT CET Biology PYQs including Applications of Biotechnology questions regularly will improve accuracy, speed, and confidence in the MHT CET 2026 exam.
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MHT CET PYQs for Applications of Biotechnology with Solutions
1.
What was the historical use of cellophane tubes in hemodialysis?- Providing structural support to dialysis machines
- Acting as semipermeable membranes
- Delivering medication to patients
- Filtering blood cells directly
2.
Which of the following techniques is commonly used to introduce herbicide resistance into plants?- RNA interference.
- CRISPR-Cas9 gene editing.
- Polymerase chain reaction (PCR).
- Agrobacterium-mediated gene transfer.
3.
To increase wool production and to improve the quality of wool, the bacterial genes concerned with biosynthesis of cysteine amino acids involved in formation of keratin protein found in wool are cloned and introduced in sheep.- cys E, cys M.
- cys F, cys G.
- cys F, cys M.
- lac y, lac a.
4.
Biopatent is granted for biological entities by _______ authority of a country.- Government
- Educational institute
- Scientist
- Business
5.
Transgenic hirudin is obtained from- Ocimum sanctum
- Brassica napus
- Potato
- Tomato
6.
Which one of the following shows the correct sequence of the path of urine in the human excretory system?- (A) Kidney → Urinary bladder → Ureter → Urethra
- (B) Kidney → Urinary bladder → Urethra → Ureter
- (C) Kidney → Urethra → Urinary bladder → Ureter
- (D) Kidney → Ureter → Urinary bladder → Urethra
7.
Annealing process of PCR is performed at \(\_\_\_\) temperature.
- 90-98°C.
- 40-60°C.
- 70-75°C.
- 20-25°C.
8.
The sex of the children will be determined by what they inherit from the:- (A) Nature
- (B) Father
- (C) Mother
- (D) Mother and father
9.
Which of the following gases is primarily responsible for eutrophication of water bodies?- (A) Nitrogen
- (B) Sulphur dioxide
- (C) Carbon dioxide
- (D) All the above
10.
Who first identified the transforming principle?- Oswald Avery.
- Frederick Griffith.
- James Watson.
- Rosalind Franklin.
11.
Identify the INCORRECT statement regarding Bt Cotton.- Dried spores of Bacillus thuringiensis are sprayed on its vulnerable leaves
- It releases toxin that causes swelling in the gut of insect that ingest its leaves
- It is a transgenic plant
- It can produce toxin due to gene introduced in it by transgenesis
12.
What percentage of the world's area does India occupy?4.4%.
6.1%.
2.4%.
9.8%.
13.
Anticoagulant hirudin is obtained from genetically modified- Seeds of Brassica napus
- Bolls of Bt cotton
- Golden Rice
- All the above
14.
Which of the following is a restriction endonuclease?- DNA polymerase
- RNA polymerase
- EcoRI
- Ligase
15.
Ti plasmid is obtained from \(\_\_\_\_\)- Escherichia coli
- Agrobacterium tumefaciens
- Bacillus subtilis
- Saccharomyces cerevisiae
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